http://hdl.handle.net/20.500.12474/21 2026-04-29T05:12:33Z http://hdl.handle.net/20.500.12474/69 Outcomes of trochanteric femoral fractures treated with proximal femoral nail: an analysis of 100 consecutive cases Korkmaz, Mehmet Fatih; Erdem, Mehmet Nuri; Dişli, Zeliha; Selçuk, Engin Burak; Karakaplan, Mustafa; Göğüs, Abdullah Purpose: In this study, we aimed to report the results of a retrospective study carried out at our institute regarding cases of patients who had suffered proximal femoral fractures between January 2002 and February 2007, and who were treated with a proximal femoral nail. Materials and methods: One hundred consecutive cases were included in the study. A case documentation form was used to obtain intraoperative data including age, sex, mechanism of injury, type of fracture according to Association for Osteosynthesis/Association for the Study of Internal Fixation (AO/ASIF) classification and the American Society of Anesthesiologists' (ASA) physical status classification (ASA grade). Clinical and radiographic examinations were performed at the time of admission and at the 6th week; subsequent visits were organized on the 3rd month, 6th month, and 12th month, and in patients with longer - follow-up and annually postoperatively. The Harris score of hip function was used, and any change in the position of the implants and the progress of the fracture union, which was determined radiologically, was noted. Results: The mean age of the patients was 77.66 years (range: 37-98 years), and the sex distribution was 32 males and 68 females. Seventy-three fractures were reduced by closed means, whereas 27 needed limited open reduction. The mean follow-up time for the study group was 31.3 months (range: 12-75 months). Postoperative radiographs showed a near-anatomical fracture reduction in 78% of patients. The Harris hip score was negatively correlated with the ASA score and patient age. No cases of implant failure were observed. Three patients died before discharge (one due to pulmonary embolism, two due to cardiac arrest), and five patients died due to unrelated medical conditions within the first 3 months of the follow-up. Conclusion: Our study showed that proximal femoral nail is a reliable fixation with good fracture union, and it is not associated with major complications in any type of trochanteric femoral fracture. 2014-04-01T00:00:00Z http://hdl.handle.net/20.500.12474/68 Does insulin, transferrin and selenous acid preparation effect chondrocyte proliferation? Gökçe, Alper; Yılmaz, İbrahim; Gökay, Nevzat Selim; Can, Levent; Gökçe, Çiğdem Objective: The aim of this study was to test the hypothesis that insulin, human transferrin, and selenous acid (ITS) preparation have positive effects on chondrocyte proliferation and morphology and investigate the biochemical and histological effects of these additive substances in different cell culture media. Methods: Human cartilage-derived cells (hCDCs) were isolated from the cartilage tissue of a 57-year-old woman diagnosed with gonarthrosis. Tissue samples were cultured in Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640. The cells' chondrogenic activities were observed. After serial passagings, cells were divided into 4 groups at the end of the 6th week. On the 14th day, proliferated cells were examined using an inverted microscope with x4, x10, x20 and x40 magnification and microphotographs were taken. Living cell quantity was determined on the first and 14th days using MTS-ELISA cell proliferation assay. Results: DMEM (without adding ITS premix solution) and RPMI-1640 containing ITS premix solution provide proliferation of the chondrogenic cells. The proliferation and viability of chondrocytes were revealed in this study in the 3rd group (DMEM solution without additives). Conclusion: It is suggested that the culture medium ingredients play crucial roles on chondrogenic proliferation in osteochondral tissue cultures. 2014-01-01T00:00:00Z http://hdl.handle.net/20.500.12474/64 A Comparison of the Effects of Neuronal Nitric Oxide Synthase and Inducible Nitric Oxide Synthase Inhibition on Cartilage Damage Gökay, Nevzat Selim; Yılmaz, İbrahim; Kömür, Baran; Demiröz, Ahu Senem; Gökçe, Alper; Dervişoğlu, Sergülen; Gökay, Banu Vural The objective of this study was to investigate the effects of selective inducible nitric oxide synthase and neuronal nitric oxide synthase inhibitors on cartilage regeneration. The study involved 27 Wistar rats that were divided into five groups. On Day 1, both knees of 3 rats were resected and placed in a formalin solution as a control group. The remaining 24 rats were separated into 4 groups, and their right knees were surgically damaged. Depending on the groups, the rats were injected with intra-articular normal saline solution, neuronal nitric oxide synthase inhibitor 7-nitroindazole (50 mg/kg), inducible nitric oxide synthase inhibitor aminoguanidine (30 mg/kg), or nitric oxide precursor L-arginine (200 mg/kg). After 21 days, the right and left knees of the rats were resected and placed in formalin solution. The samples were histopathologically examined by a blinded evaluator and scored on 8 parameters. Although selective neuronal nitric oxide synthase inhibition exhibited significant (𝑃 = 0.044) positive effects on cartilage regeneration following cartilage damage, it was determined that inducible nitric oxide synthase inhibition had no statistically significant effect on cartilage regeneration. It was observed that the nitric oxide synthase activation triggered advanced arthrosis symptoms, such as osteophyte formation. The fact that selective neuronal nitric oxide synthase inhibitors were observed to have mitigating effects on the severity of the damage may, in the future, influence the development of new agents to be used in the treatment of cartilage disorders. 2016-01-01T00:00:00Z http://hdl.handle.net/20.500.12474/60 Relationship between breast arterial calcification and lipid profile, plasma atherogenic index, Castelli's risk index and atherogenic coefficient in premenopausal women Yıldız, Abdulmelik; Seçen, Özlem; Yıldız, Cennet; Çiçekçi, Mehtap Objective: The aim of this study was to investigate the relationship between the breast arterial calcification (BAC) and the plasma atherogenic index (PAI), atherogenic coefficient (AC), Castelli risk index-I (CRI-I) and Castelli risk index-II (CRI-II). Methods: This retrospective study included 60 premenopausal women aged over 40 years with BAC on mammograms and control group of 60 women without BAC. Serum glucose, triglyceride (TG), low-density lipoproteincholesterol (LDLc), high-density lipoprotein-cholesterol (HDLc), and total cholesterol (TC), levels were measured. Lipid indices were calculated using the appropriate formula. Results: LDLc, non-HDLc levels were significantly higher, HDLc levels were siginificantly lower in patient group compared to the control group (p=0.007, p=0.027, and p=0.014, respectively). Patient group had significantly higher PAI, AC, CRI-I and CRI-II levels than the control group (p=0.003, p=0.002, p=0.002 and p=0.003, respectively). A significant positive correlation was found between BAC and PAI, AC, CRI-I and CRI-II (r=0.267 and p=0.003, r=0.282 and p=0.002, r=0.282 and p=0.002, r=0.271 and p=0.003, respectively). LDLc and non-HDLc were positively correlated whereas HDLc was negatively correlated with the BAC (r=0.188 and p =0.039, r =0.202 and p =0.027, r =-0.223 and p =0.014, respectively). Conclusion: BAC is a valuable tool for the prediction of deranged lipid profile. Dyslipidemia, PAI, AC, CRI-I and CRI-II are risk factors for the development of atherosclerosis. Our results indicate that BAC is potentially useful tool for the detection of dyslipidemia and early atherosclerosis in premenopausal women. (c) 2016 Published by Elsevier Ireland Ltd. 2016-06-01T00:00:00Z